The protective effect of North Schisandra Lignans on vascular endothelial cell oxidation injuries.

BACKGROUND In this study, the authors cultivated ECV-304 in vitro and incubated cells with H2O2, established injury models, and induced oxidized endothelial cell apoptosis. This model makes it possible to choose suitable concentrations of North Schisandra Lignans. OBJECTIVE To study the protective effects of North Schisandra Lignans on human umbilical vein endothelial cell injuries. METHODS Endothelial cell growth and proliferation activity were detected through the MTT method. The colorimetric method was used to determine superoxide dismutase (SOD) activity in the cell culture solution, as well as malondialdehyde (MDA) content in the cell. RESULTS North Schisandra Lignans noticeably decreased ECV-304 cell injury induced by H2O2. Moderate and high concentrations of North Schisandra Lignans could significantly lower MDA content and heighten SOD activity. These differences were significant compared to the H2O2 group (P< 0.05). CONCLUSIONS North Schisandra Lignans had an obvious protective effect on ECV-304 injured by H2O2$. The mechanism decreases MDA production and heightened SOD activity.